New strain of Trichoderma with increased biological activity, especially for use in agriculture as a biofungicide and method for producing this strain

摘要

The invention applies to a new Trichoderma atroviride strain with improved biocontrol activity, for use in agriculture as a biofungicide. The method of obtaining the Trichoderma atroviride strain with elevated biocontrol activity, according to the invention, is performed by modification of the amount of secretory proteins and of their secretion achieved by expression of the DPM1 gene from S. cerevisiae coding for DPM synthase, an enzyme synthesizing Dol-P-Mannose. Expression is obtained by transformation of the fungus with a vector carrying the DPM1 gene. The observed enhanced antifungal properties are a result of the DPM1 gene overexpression leading to elevated cellulolytic activity of secretory proteins and their increased amount.