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PROCESS FOR PREPARING COPPER-, ZINC- AND MANGANESE-ENRICHED BIOPREPARATION

机译:制备富铜,富锌和富锰生物素的过程

摘要

The present invention relates to a process for preparing a biopreparation based on copper, zinc and manganese, by microbial conversion. According to the invention, the process consists in selecting the cells of S. Cerevisiae which have resisted to various concentrations of ZnSO4 - 10,000 ppm (1 % solution), CuSO4 - 10, 000 ppm (1% solution), MnSo4 - 12, 500 ppm (1% solution), there being obtained the laboratory pre-inoculum with the cell viability of 2,14X 10^4 cfu/ml and then the laboratory inoculum which was used in a percentage of 15% as seeding material for a well-established fermentation medium, with the correction of the pH during the culturing within the 5 - 5.5 range, obtained the biopreparation which is separated by filtration, the biomass being purified, pasteurized and dried, finally reaching a concentration of yeast biomass of 14 g/l, which exhibits a high degree of incorporation of the minerals Cu (1016 ppm), Zn (1123.8 ppm) and manganese (331 ppm).
机译:本发明涉及通过微生物转化制备基于铜,锌和锰的生物制剂的方法。根据本发明,该方法在于选择对各种浓度的ZnSO 4-10,000 ppm(1%溶液),CuSO 4-10、000 ppm(1%溶液),MnSo4-12,500的各种浓度的酿酒酵母细胞。 ppm(1%溶液),得到细胞活力为2,14X 10 ^ 4 cfu / ml的实验室接种物,然后以15%的百分比用作实验室接种物的接种物,建立的发酵培养基,在培养过程中将pH值校正在5-5.5范围内,获得了生物制剂,将其过滤分离,对生物质进行纯化,巴氏灭菌和干燥,最终使酵母生物质浓度达到14 g / l ,其中的矿物质铜(1016 ppm),锌(1123.8 ppm)和锰(331 ppm)具有很高的结合度。

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