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REAGENTS AND METHODS FOR IMPROVING REPRODUCIBILITY AND REDUCING MISPRIMING IN PCR AMPLIFICATION
REAGENTS AND METHODS FOR IMPROVING REPRODUCIBILITY AND REDUCING MISPRIMING IN PCR AMPLIFICATION
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机译:在PCR扩增中提高重现性和减少错配的试剂和方法
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摘要
An additive for preventing mispriming in polymerase chain reaction (PCR) amplifications and assays comprising a hairpin oligonucleotide having a stem duplex greater than six nucleotides in length and a stabilized stem terminus. The additive improves PCR amplifications, including LATE-PCR amplifications when added to initial amplification reaction mixtures. It can be included in oligonucleotide sets and in kits for PCR amplification and assays.
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