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P450 monooxygenases of cytochrome thermophilic bacteria

机译:细胞色素嗜热菌的P450单加氧酶

摘要

Cytochrome P450 monooxygenase, characterized in that it exhibits an amino acid sequence according to SEQ ID NO: 2 and functional equivalents thereof which differ from SEQ ID NO: 2 by 1 to 30 additions, substitutions, deletions and / or inversions of amino acids and wherein the modification of the sequence determined by use of the reference substrate ß-ionone, does not change monooxygenase activity by more than ± 90%, where the activity is determined under standardized conditions, ie from 0.1 to 0 , 5 M of the substrate, pH 6 to 8 and T = 60 -70 ° C.
机译:细胞色素P450单加氧酶,其特征在于它具有SEQ ID NO:2所示的氨基酸序列及其功能等同物,与SEQ ID NO:2的区别在于氨基酸增加,取代,缺失和/或倒置1至30个,其中通过使用参比底物β-紫罗兰酮测定的序列修饰,单加氧酶活性的变化不会超过±90%,在标准条件下,即从0.1到0,5 M底物,pH 6至8和T = 60 -70°C。

著录项

  • 公开/公告号ES2382027T3

    专利类型

  • 公开/公告日2012-06-04

    原文格式PDF

  • 申请/专利权人 BASF SE;

    申请/专利号ES20010987798T

  • 申请日2001-10-16

  • 分类号C12N15/09;C12N15/53;C12M1/40;C12N1/15;C12N1/19;C12N1/21;C12N5/10;C12N9/02;C12P1/04;C12P7/00;C12R1/01;

  • 国家 ES

  • 入库时间 2022-08-21 17:21:09

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