首页>
外国专利>
P450 monooxygenases of cytochrome thermophilic bacteria
P450 monooxygenases of cytochrome thermophilic bacteria
展开▼
机译:细胞色素嗜热菌的P450单加氧酶
展开▼
页面导航
摘要
著录项
相似文献
摘要
Cytochrome P450 monooxygenase, characterized in that it exhibits an amino acid sequence according to SEQ ID NO: 2 and functional equivalents thereof which differ from SEQ ID NO: 2 by 1 to 30 additions, substitutions, deletions and / or inversions of amino acids and wherein the modification of the sequence determined by use of the reference substrate ß-ionone, does not change monooxygenase activity by more than ± 90%, where the activity is determined under standardized conditions, ie from 0.1 to 0 , 5 M of the substrate, pH 6 to 8 and T = 60 -70 ° C.
展开▼
机译:细胞色素P450单加氧酶,其特征在于它具有SEQ ID NO:2所示的氨基酸序列及其功能等同物,与SEQ ID NO:2的区别在于氨基酸增加,取代,缺失和/或倒置1至30个,其中通过使用参比底物β-紫罗兰酮测定的序列修饰,单加氧酶活性的变化不会超过±90%,在标准条件下,即从0.1到0,5 M底物,pH 6至8和T = 60 -70°C。
展开▼