Method for increasing resistance to fungi in plant biotropic

摘要

Method for generating and raising a resistance to at least one biotrophic fungus genus Phakopsora ina plant or part of a plant, in which case the part of a plant comprises a tissue or a cell, the cualcomprende steps: (a) raise the amount or function of the protein of at least one inhibitor protein - 1 Bax (BI1) in the plant or at least a portion of a plant from the amount or function of protein in the starting plant or parts thereof, encuyo case ( i) plant cells stably transformed with a recombinant expression cassette containing unasecuencia nucleic acid encoding a BI1 protein in functional linkage with a promoter, in which forwarder case is heterologous to the nucleic acid sequence encoding BI1 protein; (ii) the plant is regenerated from plant cells; and (iii) the nucleic acid sequence encoding the BI1 protein is expressed in an amount, in at least one tissue opor a time sufficient to generate or enhance resistance to fungi on the plant against biotrophic unhongo genus way Phakopsora, and (b) selecting the plant or plant part in which compared to the starting plant or partegenera or increases resistance against at least one biotrophic fungus of the genus Phakopsora, in which case the proteínaBI1 is encodes a polypeptide comprising at least one sequence selected from the group componede: (a) the sequences according to SEQ ID NO: 2, 4, 6, 8, 10, 12, 14, 16, 18, 20, 22, 24 , 26, 28, or 38; and (b) sequences having an identity of at least 70% to one of the sequences according to SEQ ID NO: 2, 4, 6, 8, 10,12, 14, 16, 18, 20, 22, 24, 26, 28 or 38, in which case the plant is soybean.