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A FAST METHOD OF TRANSFORMING COMPETENT CELLS
A FAST METHOD OF TRANSFORMING COMPETENT CELLS
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机译:一种快速转化感受态细胞的方法
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摘要
The invention relates to a fast method of transforming competent cells, comprising: a. mixing a plasmid DNA with the competent cells suspending within ionic solution to form a mixture; b. plating the mixture on a warm selective medium; and c. culturing the mixture on the medium; wherein the ionic solution comprises divalent cation selected from the group consisting of Ca2+, Mg2+, Mn2+, Zn2+, Cu2+, Cd2+, Fe2+, Sr2+ and Co2+, and provided that the ionic solution does not include Ca2+ alone.
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