A FAST METHOD OF TRANSFORMING COMPETENT CELLS

摘要

the present invention comprises: (a) plasmid DNA from the ion solution in the competent cell suspension and mixed to a mixture of to prepare ; (b) the plated on selective media to warm the mixture ; And (c) comprises the step of culturing the mixture on the culture medium , relates to a rapid transformation of the competent cell method , the ion solution is Ca ^{2 +} , Mg ²⁺ , Mn ^{2 +} , Zn ^{2 +} , Cu ^{2 +} , Cd ^{2 +} , Fe ^{2 +} , Sr ²⁺ , and Co ^{2 + 2 is selected from the group consisting of} comprises a cation , wherein the ionic solution is Ca ²⁺ relates to a transformation method , characterized in that only that does not contain alone .