ALLOSTERIC REGULATION OF KINASE ACTIVITY

摘要

The present invention relates to fusion proteins comprising a kinase and a ligand binding domain inserted in the kinase in or near the catalytic domain to produce a ligand-dependent kinase. The invention further relates to polynucleotides and vectors encoding the fusion proteins, cells comprising the fusion proteins, and methods of using the fusion proteins to study kinase function and analyze cellular activity, as well as diagnostic and therapeutic methods.