METHOD FOR SIMULTANEOUS ACHIEVEMENT OF BACTERIOLYSIS OF ACID-FAST BACTERIUM AND SEPARATION OF NUCLEIC ACID FROM THE BACTERIUM

摘要

Disclosed is a method for achieving the bacteriolysis of an acid-fast bacterium and the separation of a nucleic acid from the bacterium simultaneously, with high efficiency and in a simple manner. The method is characterized by comprising preparing a mixed solution containing a chaotropic salt and the acid-fast bacterium and repeating the suction of the mixed solution and the ejection of the mixed solution using a chip having, melt-adhered to the inside thereof, a continuous body having a pore structure, wherein the pore structure has a pore diameter of 10 to 100 μm. In the method, it is preferred to repeat the suction and the ejection at least three times.