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Transposable elements in rice and methods of use

机译:大米中的转座因子及其使用方法

摘要

Disclosed are isolated transposable elements, or isolated DNA sequences which encode a transposase protein or a portion of a transposase protein. The isolated transposable elements or the isolated DNA sequences are members of the mPing/Pong family of transposable elements. The invention also relates to a purified transposase protein, or peptide fragments thereof, encoded by such DNA sequences. Such transposable elements are useful in applications such as the stable introduction of a DNA sequence of interest into a eukaryotic cell. The sequence information disclosed herein is useful in the design of oligonucleotide primers which are useful for the isolation of related members of the mPing/Pong family of transposable elements, or for the detection of transpositions of the transposable elements.
机译:公开了分离的转座因子,或编码转座酶蛋白或部分转座酶蛋白的分离的DNA序列。分离的转座因子或分离的DNA序列是mPing / Pong转座因子家族的成员。本发明还涉及由此类DNA序列编码的纯化的转座酶蛋白或其肽片段。这样的转座元件可用于诸如将目的DNA序列稳定地引入真核细胞的应用中。本文公开的序列信息可用于设计寡核苷酸引物,所述寡核苷酸引物可用于分离可转座元件的mPing / Pong家族的相关成员,或用于检测可转座元件的转座。

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