BIOCHEMICAL REACTION SYSTEM, BIOCHEMICAL REACTION SUBSTRATE, PROCESS FOR PRODUCING HYBRIDIZATION SUBSTRATE AND HYBRIDIZATION METHOD

摘要

PROBLEM TO BE SOLVED: To provide a biochemical reaction apparatus capable of performing a biochemical reaction by using a substrate, the substrate for the biochemical reaction such as a DNA (deoxyribonucleic acid) chip, a hybridization method of hybridizing a nucleotide chain, and a biochemical reaction apparatus capable of performing hybridization at a high speed relative to a substrate manufacturing method for the hybridization wherein the nucleotide chain for a probe is immobilized.;SOLUTION: As for the substrate 1 for bioassay, the main surface similar to an optical disk such as a CD has an annular flat shape. The substrate 1 is driven rotatively around a center hole 2, and the substrate 1 has the surface 1a on which a plurality of wells 8 are formed, which are fields for a hybridization reaction between a probe DNA (a nucleotide chain for detection) and a sample DNA (a target nucleotide chain). A transparent electrode film 4 is formed on the lower layer of the wells 8 on the substrate 1. At the hybridization time, an external electrode 18 is brought close from the upper surface 1a side of the substrate 1, and an alternating-current power is applied between the transparent electrode film 4 and the external electrode 18, to thereby give an alternating-current field in the vertical direction to the substrate 1.;COPYRIGHT: (C)2005,JPO&NCIPI