Eukaryotic amadoriase having excellent thermal stability, gene and recombinant DNA for the eukaryotic amadoriase, and process for production of eukaryotic amadoriase having excellent thermal stability

摘要

The object is to overcome the defects associated with thermal stability in a conventional eukaryotic amadoriase and to provide a eukaryotic amadoriase having excellent thermal stability for use as an enzyme for clinical diagnosis of diabetes or an enzyme sensor. Disclosed are: a eukaryotic amadoriase which is prepared by introducing a mutation into DNA encoding a eukaryotic amadoriase derived from a microorganism belonging to the genus Coniochaeta or Eupenicillium so as to introduce a substitution into a specific amino acid residue in the eukaryotic amadoriase, thereby overcoming the defect associated with thermal stability; a gene or recombinant DNA for the eukaryotic amadoriase; and a process for production of a eukaryotic amadoriasehaving excellent thermal stability.