SIGNAL SEPARATOR, METHOD FOR DETERMINING OUTPUT SIGNALS ON THE BASIS OF MICROPHONE SIGNALS, AND COMPUTER PROGRAM

摘要

A method for determining the concentration of nucleic acids in a sample in a microfluidic device, comprising the steps of:a) introducing the sample into a first chamber,b) performing a cyclically amplification reaction, which can be carried out for the amplification of nucleic acids for a predetermined number of cycles,c) transferring a defined volume, which is a fraction of the volume of the first chamber, and which is amplified nucleic acids, in a second chamber and replacement of the transferred defined volume with unused reagents for the amplification reaction,d) determining the concentration of the amplified nucleic acids in a second chamber, comprising means for determining the concentration, ande) repeating steps b) – d), to in the second chamber a concentration of the amplified nucleic acids is determined, which is located in a predetermined area,wherein the predetermined area of the measuring range of the device for determining the concentration is.