A METHOD FOR THE QUANTITATIVE GLYCOSYLATION ANALYSIS OF PROTEINS

摘要

A method for the quantitative determination of glycosylation of a selected protein in solution comprising the steps: providing a conjugate of a nucleic acid or nucleic acid analogues, such as LNA and PNA hybrids, and a compound having an affinity to a carbohydrate moiety on glycosylated protein, such as a lectin binding to sialic acid attached to terminal galactose in (α-2,6), and (α-2,3), linkages; said conjugate is contacted with a protein which is suspected to be glycosylated and is called analyte-protein herein below; the conjugate is detected by a nucleotide hybridization depending technique which is employed for detecting the conjugate.