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ENTEROCOCCUS AND FECAL BACTEROIDES FOR RAPID WATER QUALITY ASSESSMENT
ENTEROCOCCUS AND FECAL BACTEROIDES FOR RAPID WATER QUALITY ASSESSMENT
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机译:肠球菌和粪便中的杀菌剂用于快速水质评估
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摘要
The present invention is drawn to methods and compositions for the rapid assessment of fecal indicator bacteria in a sample. Provided herein are novel primer and probe compositions for use in detecting the presence of these organisms in a sample, particularly using quantitative PCR methods. Provided herein are novel oligonucleotide primers and probes, including the primers set forth in SEQ ID NOS: 1- 4, 7 and 8, the novel oligonucleotide probe sequences set forth in SEQ ID N0S:5, 6, and 9, and methods for using these primers and probes for the detection and/or quantification of fecal indicator bacteria, particularly Enterococcus spp. and fecal Bacteroides spp., or a specimen processing control, in a sample.
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机译:本发明涉及用于快速评估样品中粪便指示剂细菌的方法和组合物。本文提供了新颖的引物和探针组合物,用于检测样品中这些生物的存在,特别是使用定量PCR方法。本文提供了新颖的寡核苷酸引物和探针,包括SEQ ID NO:1-4、7和8所示的引物,SEQ ID NO:5、6和9所示的新型寡核苷酸探针序列,以及使用方法这些引物和探针,用于检测和/或定量粪便指示菌,尤其是肠球菌 I> spp。样品中的粪便中的I. Bacteroides I> spp。或样本处理对照。
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