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ANALYSIS OF AMINO ACIDS AND AMINE-CONTAINING COMPOUNDS USING TAGGING REAGENTS AND LC-MS WORKFLOW
ANALYSIS OF AMINO ACIDS AND AMINE-CONTAINING COMPOUNDS USING TAGGING REAGENTS AND LC-MS WORKFLOW
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机译:标记试剂和LC-MS工作流程分析氨基酸和含胺化合物
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摘要
A plurality of mass differential tagging reagents is used to label amine functionality in amine-containing compounds. The labeled analytes have distinct retention times on a reversed phase column, and distinct masses. Under high energy collision, reporter groups can be generated and the intensity or the peak area detected for each reporter group can be used for quantitation. One exemplary set of reagents includes a set of three different mass differential reagents comprising tagging weights of (140) atomic mass units, (144) atomic mass units, and (148) atomic mass units, respectively, with reporter groups of (113, 117, and 121) atomic mass units, respectively. A package including each of the mass differential reagents is also provided and can include separate respective containers, for example, one for each of the different reagents. The package can also include one or more standards each comprising a respective known concentration of a respective known amine-containing compound.
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