Preparation method of positive control for diagnosis of RSV viruliferous insect, positive control agent, and diagnosis method using the same

摘要

PURPOSE: A method for manufacturing a positive control group for diagnosing RSV viruliferous insect is provided to enhance diagnosis reliability. CONSTITUTION: A method for manufacturing a positive control group for diagnosing RSV viruliferous insect comprises: a step of inoculating Laodelphax striatella to a RSV viruliferous infected plant; a step of mixing and pulverizing Laodelphax striatella; a step of searching viruliferous insect rate and pulverizing Laodelphax striatella when the viruliferous insect rate is more than 30%; and a step of pulverizing at least 10 viruliferous insects. A diagnosis method is performed by ELISA(Enzyme-linked immunosorbent assay) or RT-PCR(reverse transcriptase polymerase chain reaction).