PURPOSE: A method for crystallizing heterodimer of human Mst1 SARAH domain and Rassf5(Nore1) SARAH domain is provided to enable X-ray analysis and to continuously obtain three dimensional data.;CONSTITUTION: A method for crystallizing heterodimer of human recombinant Mst1 SARAH domain and Rassf5(Nore1) SARAH domain comprises a step of crystallizing the heterodimer by a hanging-drop vapour diffusion method using a reservoir solution containing 1.0-2 M ammonium sulfate as a precipitating agent, 0.01-1 M CAPS buffer solution, and sodium chloride(NaCl) as a salt solution. The reservoir solution contains 0.01-1 M LiSO_4. The hanging-drop vapour diffusion method comprises a step of reacting at 15-25°C for one day to one week.;COPYRIGHT KIPO 2012
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机译：目的：提供一种结晶人Mst1 SARAH结构域和Rassf5（Nore1）SARAH结构域的异二聚体的方法，以进行X射线分析并连续获得三维数据。 Rassf5（Nore1）SARAH结构域包括使用包含1.0-2 M硫酸铵作为沉淀剂，0.01-1 M CAPS缓冲溶液和氯化钠（NaCl）的储液通过悬滴气相扩散法结晶异二聚体的步骤。 ）作为盐溶液。储液中含有0.01-1 M LiSO_4。悬滴蒸气扩散法包括在15-25℃下反应一天到一周的步骤。COPYRIGHTKIPO 2012
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