首页> 外国专利> NEW SNPS AND GENOTYPE ASSOCIATED TO MEAT QUALITY ACCORDING TO VARIATION, AND METHOD FOR IDENTIFICATION OF HIGH QUALITY PIGS

NEW SNPS AND GENOTYPE ASSOCIATED TO MEAT QUALITY ACCORDING TO VARIATION, AND METHOD FOR IDENTIFICATION OF HIGH QUALITY PIGS

机译:根据变化与肉品质量相关的新SNPS和基因型,以及鉴定高品质猪的方法

摘要

PURPOSE: An SNP marker associated with pork meat quality is provided to early select and grow excellent breeding pigs and to produce competitive pork. CONSTITUTION: A primer set for selecting pork of high quality is an allele specific primer(oligo1) set having a base of sequence number 1 or an allele specific primer(oligo2) having a base of sequence numbers 38-74. An SNP marker is prepared by amplifying bases of sequence numbers 1-37 by the primer set or by PCR of the primer set. A method for selecting a breeding pig with high quality comprises: a step of preparing gDNA from a pork sample; a step of performing PCR using the oligo 1 or oligo 2; a step of detecting a reactant by scanning veracode beadplate by an illumine goldengate method; and a step of detecting and classifying genotype of the scanned result using BeadStudio software(Illumina).
机译:目的:提供与猪肉品质相关的SNP标记,以供早期选择和培育优良种猪并生产具有竞争力的猪肉。构成:用于选择优质猪肉的引物组是具有序列号为1的碱基的等位基因特异性引物(oligo1)或具有序列号为38-74的碱基的等位基因特异性引物(oligo2)。通过用引物组或通过引物组的PCR扩增序列号1-37的碱基来制备SNP标记。一种选择优质种猪的方法,包括:从猪肉样品中制备gDNA的步骤;使用oligo 1或oligo 2进行PCR的步骤;通过照亮金门方法扫描veracode珠板来检测反应物的步骤;以及使用BeadStudio软件(Illumina)检测和分类扫描结果的基因型的步骤。

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