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SNP marker associated to meat quality according to variation, and method for identification of high quality pigs using same
SNP marker associated to meat quality according to variation, and method for identification of high quality pigs using same
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机译:与变异有关的与肉质相关的snp标记,以及使用该标记鉴定高品质猪的方法
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摘要
PURPOSE: An SNP marker associated with pork meat quality is provided to early select and grow excellent breeding pigs and to produce competitive pork. CONSTITUTION: A primer set for selecting pork of high quality is an allele specific primer(oligo1) set having a base of sequence number 1 or an allele specific primer(oligo2) having a base of sequence numbers 38-74. An SNP marker is prepared by amplifying bases of sequence numbers 1-37 by the primer set or by PCR of the primer set. A method for selecting a breeding pig with high quality comprises: a step of preparing gDNA from a pork sample; a step of performing PCR using the oligo 1 or oligo 2; a step of detecting a reactant by scanning veracode beadplate by an illumine goldengate method; and a step of detecting and classifying genotype of the scanned result using BeadStudio software(Illumina).
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