首页> 外国专利> MANUFACTURING METHOD OF TRANSFORMED POTATO PLANT WITH MULTIFARIOUSNESS VIRUS RESISTANCE AND A TRANSFORMED POTATO PLANT MANUFACTURED BY USING THE SAME

MANUFACTURING METHOD OF TRANSFORMED POTATO PLANT WITH MULTIFARIOUSNESS VIRUS RESISTANCE AND A TRANSFORMED POTATO PLANT MANUFACTURED BY USING THE SAME

机译:具有多种抗病毒性的转化马铃薯的制造方法以及使用该方法制造的转化马铃薯

摘要

PURPOSE: A manufacturing method of transformed potato plant with multifariousness virus resistance and a transformed potato plant manufactured by using the same are provided to increase potato productivity and to be used as a virus resistance parent.;CONSTITUTION: A transformed potato plant is obtained by being transformed into an expression vector pK7GWIWG 2 which includes a gene fusing more than 3 kinds of virus genes selected from potato mop-top virus gene of the sequence number 5, PVY(Potato virus Y) gene of the sequence number 1(SEQ ID NO:1), PLRV(Potato leafroll virus) gene of the sequence number 2,PVA(Potato virus A) gene of the sequence number 3, or TRV(Tobacco rattle virus) gene of the sequence number 4 in sense and anti-sense direction. A manufacturing method of the multifariousness virus resistant transformed potato plant comprises the following steps: transforming Agrobacterium tumefaciens into an expression vector pK7GWIWG 2(I)/ YLRATRPM including YLRATRPM gene of the sequence number 6; and transforming potato cells by cocltivating agrobacterium and potato cells.;COPYRIGHT KIPO 2013
机译:目的:提供一种具有多重抗病毒性的转化马铃薯植物的生产方法,以及使用该方法生产的转化马铃薯植物,以提高马铃薯的生产力,并用作抗病毒亲本。转化成表达载体pK7GWIWG 2,该载体包含融合了选自序列号5的马铃薯拖把病毒基因的3种以上病毒基因的基因,序列号1的PVY(马铃薯病毒Y)基因(SEQ ID NO: 1)在有义和反义方向上,序列号为2的PLRV(马铃薯卷叶病毒)基因,序列号为3的PVA(马铃薯甲病毒)基因,或序列号为4的TRV(烟草花鼓病毒)基因。一种抗多种病毒的转化马铃薯植物的制备方法,包括以下步骤:将根癌土壤杆菌转化为包含序列号为6的YLRATRPM基因的表达载体pK7GWIWG 2(I)/ YLRATRPM。并通过土壤杆菌和马铃薯细胞的克隆转化马铃薯细胞。; COPYRIGHT KIPO 2013

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