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Method of judging economic genetic traits by Calcium Sensing Receptor in chicken

机译:用钙受体判断鸡经济遗传性状的方法。

摘要

The present invention relates to a method for determining the genetic economic trait of a chicken by using a calcium sensor receptor gene of chicken, (a) for amplification corresponding to the genomic gene of the chicken sample and the calcium sensor receptor (CaSR) gene derived from chicken Amplifying the calcium sensor receptor gene by PCR using a primer; (b) SNPs of the amplified calcium-sensing receptor genes to the restriction enzyme Nco I by RFLP method, and then the SNP for each sample according to the difference in allelic type due to the missense mutation (base substitution) of the calcium-sensing receptor in the gene processing product Detecting a labeling factor; And (c) predicting the gestational age, egg weight, spawning number or body weight of the chicken from the detected SNP labeling factor.;It is believed that the present invention can play a very important role in improving the economic quality of chickens.;Chicken, calcium receptor, SNP, improved
机译:本发明涉及一种通过使用鸡的钙传感器受体基因来确定鸡的遗传经济性状的方法,(a)用于扩增对应于鸡样品的基因组基因和钙传感器受体(CaSR)基因的基因来自鸡的引物通过PCR扩增钙传感器受体基因; (b)通过RFLP方法将扩增的钙敏感受体基因的SNPs导入限制酶Nco I ,然后根据由于错义突变引起的等位基因类型的差异,每个样品的SNPs(碱基基因加工产品中钙敏感受体的取代);检测标记因子; (c)从检测到的SNP标记因子预测鸡的胎龄,蛋重,产卵数或体重。相信本发明在改善鸡的经济质量方面可以起非常重要的作用。鸡肉,钙受体,SNP,改良

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