Transgenic cloned cow carrying a knock-out prion-encoding gene and method for producing the same

摘要

The present invention relates to a method for producing a transgenic cloned animal that is resistant to a specific disease in which a gene targeting and introduction technique and a somatic cloning technique are combined, and a cloned animal produced by the method.;Specifically, the method for producing a transgenic cloned cow resistant to mad cow disease according to the present invention targets a gene encoding a prion of a bovine-derived somatic cell to produce a transgenic somatic cell, and introduces the deionized egg into a bovine-derived denuclear egg. Constructing the targeted nuclear transfer embryos and transplanting them into surrogate mothers to produce mad cow disease resistant cattle.;In addition, the transgenic nuclear transfer embryos and cloned cows of the present invention are characterized by having a normal prion hit and resistant to mad cow disease.;Transformation clone, somatic cloning, mad cow disease, prion gene hit, mad cow disease resistance