Generation composition for induced pluripotent stem cells with oxysterols and WWP2 siRNA treatment and method of manufacturing induced pluripotent stem cells using the same

摘要

The present invention is a) oxy sterols (Oxysterol); And b) a method of producing an embryonic stem cell-like cells using the composition for inducing the de-differentiation of a similar cells from somatic cells comprising a siRNA and WWP2 them. The present invention as described above is oxy sterols (oxysterol; hydroxycholesterol) of the upper adjustment factor Shh analog (analog) of Bmi1 using WWP2 siRNA, while the induction of the cells, such as neural stem cells were treated in the suppression of expression of Oct4 inhibiting the expression of WWP2 to block the inhibition of expression of Oct4, presents a new induced de-differentiation method that are not transgenic for the de-differentiation by preparing embryonic stem cell-like cells through them, and the embryonic stem cells prepared by the present invention that Similar cells can be brought before the multipotent cells such as embryonic stem effectively used for various diseases. ;