Transformant expressing two kinds of ligninases, method for preparing the transformant and method for degrading endocrine disruptor by using the transformant

摘要

The present invention 2 jong lignin degrading enzyme expression transformant, the recombinant vector according to his production method and characterized in that relates to the environmental hormone decomposing method using the same, specifically to express a manganese peroxidase gene and laccase gene at the same time than the pMnP-gLac and the method of manufacture; The recombinant vector according to gLac pMnP-transformed characterized by mechanical switches of the charge and mushroom or mushrooms glue transfected body and a method of manufacture; And a method for decomposing a lignin or environmental hormone by using the transformant. ; This is due to the recent widespread use of disposable containers, such as the environment, it accumulates in the body of a human hormone problem that causes the disease of infertility (不 姙), such as birth defects or cancer by interfering with normal hormone action in the body can occur. This lignin mushroom called white rot fungi with enzymes are used for the degradation of environmental hormones. However, transformant of the conventional white rot fungus was substantially lacking the disadvantages as biological resolution transformant inserted only one of the laccase gene dehydratase or manganese peroxidase. ; This invention provides a transformant by introducing the recombinant vector laccase and manganese peroxidase dehydratase gene is expressed at the same time to maximize the resolution of environmental hormones such as bisphenol A and benzyl butyl phthalate. ; Transgenic, white rot fungi, lignin, lacquers Ajay, manganese peroxidase, winter mushroom umbrella, glue mushrooms, mushroom charging machines, endocrine disruptors