A method for quantifying activity of proteolytic enzymes with different types of active sites capable of cleaving immunoglobulin IGA, IGM, IGG

摘要

FIELD: medicine.;SUBSTANCE: method involves tray coating with a bacterial antigen of any representative of an opportunistic microflora, coating of a sorbed antigen with IgG, IgM and IgA antibodies, a proteolytic reaction by incubation in tray wells of an analysed biological fluid together with inhibitors of active centres of the enzymes (serine, cysteine, metal-dependent), washing of the reaction products and calculation of enzymatic activity with appropriate type of the active centre.;EFFECT: invention provides simultaneous measurement of activity of proteinase of various origin and of various types of the active centres.;4 tbl, 4 ex