WAY EXPRESS assess the degree of aneuploidy BASED ON NUCLEAR morphometry peripheral blood lymphocytes of human, cultured under conditions cytokinetic UNIT, IN identify potential genotoxic activity SUBSTANCES AND ENVIRONMENTAL FACTORS

摘要

FIELD: medicine.;SUBSTANCE: invention refers to medicine, namely cytogenetics and may be used for instant assessment of a degree of potential genotoxic substance activity and environmental factors by the presence of aneuploidy in human peripheral blood lymphocytes formed by culture in cytogenetic block. That is ensured by culture of human blood lymphocytes in cytogenetic block in the presence of an analysed substance. Cells are fixed. Cytogenetic preparations are prepared and stained. Images of 100 cells containing two separate nuclei and having no visible, including genetic injures are recorded after staining for each preparation with the use of an optical microscope with an integrated digital camera at magnification 10×40. These images are used to derive a sum and a relation of nuclei areas in each such cell. The derived values are used for calculating medians, upper and lower quartiles, their relation and difference. The preparations are statically compared by these parameters. If considerable differences are found at a significance level of p≤0.05, as well as a dose response is observed, ability for the analysed substance to induce aneuploidy associated with potential genotoxic activity is stated.;EFFECT: invention provides reducing time and simplifying procedure of assessing a degree of aneuploidy in the analysis system of genotoxic activity of factors of any nature.;7 dwg, 2 ex