METHOD FOR DIFFERENTIATION OF VIBRIO CHOLERAE O139 STRAINS SERO GROUPS ON ALKYL SULPHATIC ACTIVITY

摘要

The method of differentiation of strains of Vibrio cholerae O139 serogroup by alkyl sulfatase activity, including the use of sodium dodecyl sulfate as a substrate in a nutrient medium, characterized in that they create a synthetic medium for the preparation of which per 100 ml is necessary: 0.5 g of sodium chloride, 2 g of agar, 0.002 g of bromothymol blue indicator, then the components are dissolved in 0.01 M Tris HCl buffer, having a pH of 7.8 and subjected to boiling for 30 minutes, obtaining a molten mass into which 0.1 g of sodium dodecyl sulfate substrate is introduced, then about The agar cultures under study are inoculated onto the obtained synthetic medium, followed by incubation at 37 ° C for 24-48 hours, after which they are differentiated by the alkyl sulfatase activity of the serogroup O139 cholera vibrios, which hydrolyze the sodium dodecyl sulfate substrate; therefore, agar sectors around the seed are clearly visible turbid - white zones 2-10 mm in size, which confirms the toxigenicity of the strain and its epidemiological significance, and if there are no such zones, they conclude that the strain is non-toxic and its epidemic insignificance.