Devices for direct electrochemical detection of DNA molecules NEPRAYMIROVANNOGO SYNTHESIS BASED pH-sensitive sensors

摘要

The invention relates to a biosensor assay device s.oblastyu applications in molecular biology, molecular biotechnology, bionanotechnologies, medicine, in addressing the food industry, agriculture, identification, biosafety. The apparatus performs direct that does not use tags, registration nepraymirovannogo synthetic DNA molecules, which is performed in the simultaneous presence in the reaction medium of three components - a DNA polymerase and deoxynucleoside triphosphates nickase. The synthesis is shown in isolation protons effect during the enzymatic reaction containing these components. The apparatus may be used to assess the presence in a sample of DNA synthesis enzyme - polymerase, the presence of deoxynucleoside triphosphates in the sample, and the presence of the enzyme-inducer of DNA synthesis nikuyuschey endonuclease (nickase). The apparatus can be used for registering (detecting) as an nepraymirovannogo (non-specific) and specific synthesis of DNA molecules of any nature - warmblooded cells, the microbial cells. The device can be used in molecular biology, molecular diagnostics to solve problems of fundamental and applied plan.