Devices for direct electrochemical detection of DNA SYNTHESIS OF KINETIC CHARACTERISTICS ON THE BASIS OF pH-sensitive SENSOR

摘要

Utility model performs registration kinetic characteristics of DNA synthesis based on the registration of the change in pH during the synthesis reaction. The apparatus comprises a pH-sensitive field effect transistor (measuring electrode, as which may be used in any other semiconductor or nepoluprovodnikovy pH-sensitive sensor), coupled with the flow cell, the mode control unit operation field effect transistor, the recording unit of the FET signals (XY recorder and / or computer), a reference electrode, a source of wash solution, a computer controlled peristaltic pump, a reaction block, which is charged with the reaction mixture and the cat ory placed in thermostatic block, solution block for measuring a reference value pH, prior passage of the synthesis reaction, the drain container for receiving the waste solution. The apparatus produces registration reaction kinetics of DNA synthesis based on the registration value of the solution pH, flowing through the measuring cuvette FET. The reaction nepraymirovannogo synthesizing DNA molecules using three components - a DNA polymerase nikuyuschaya endonuclease (nickase) and deoxynucleoside triphosphates (dNTPs). The apparatus can be used for recording / detection as nepraymirovannogo (non-specific) and specific synthesis of DNA molecules of any nature - warmblooded cells of microorganisms.; The invention relates to a biosensor assay device with the scope of molecular biology and biotechnology, bionanotechnologies, medicine, food industry, agriculture, biosafety, establishing identity.