METHOD OF DETERMINING BLOOD LIPOPROTEIN FRACTIONS IN PATIENTS WITH ISCHEMIC HEART DISEASE

摘要

FIELD: medicine.;SUBSTANCE: patient's blood serum is processed for 1 h in darkness at 40°C with Sudan B dye solution, agarose solution is added, heated to 55°C mixture is introduced into hollow in agarose gel with volume 4×20×10 mm, electrophoregram is fixed, dried, densitometry is carried out, to 0.3 ml of patient's blood serum sample additionally added is 0.1 ml of 0.1% triton X-100 solution and incubated for 15 min at 20°C, mixture is mixed by method of shaking 120 times per 1 min, and disintegration of lipoproteins is carried out.;EFFECT: method application makes it possible to determine maximally possible intensive minor fractions of blood LP, which increases its sensitivity and accuracy.;3 dwg, 1 ex