METHOD OF PRODUCING ENTOMOPATHOGENIC NEMATODE-BASED BIOLOGICAL AGENTS

摘要

FIELD: chemistry.;SUBSTANCE: invention relates to microbiological industry. The method involves filling bioreactors with a culture medium into which, after sterilisation and cooling, a liquid culture of symbiotic bacteria is inoculated and nematodes are inoculated therein after 48-hour exposure. The bioreactors re put into temperature-controlled chambers for development of a nematode-bacterial complex. The bioreactor is made in form of a separate polypropylene sack measuring 700×400 mm with 186 through holes with diameter of 0.5 mm on each side of the sack, at a distance of 190 mm from the top part of the sack and on an area of 1500 mm² with height of 50 mm and 300 mm width of the sack.;EFFECT: method increases output of biomass and invasive activity of nematodes.;3 dwg, 2 tbl, 1 ex