METHOD FOR HUMAN GENOTYPE DETERMINATION BY 185 GENE BRCA1 (185delAG) POSITION POLYMORPHISM

摘要

FIELD: medicine.;SUBSTANCE: invention may be used for human genotype determination by 185 gene BRCA1 (185delAG) position polymorphism. The method consists in using allele-specific primers with real-time PCR values recording with using fluorescence marked probes. A reaction mixture contains the primers different for each allele, the primer and the probe common for two alleles, and also the common fluorescent probe.;EFFECT: invention provides cutting expenses for genetic researches and making them more available due to the use of standard equipment and only one marked probe.;2 dwg