METHOD OF GENERATING ANTIGEN-SPECIFIC OF CYTOTOXIC CELLS WITH ANTICANCER ACTIVITY

摘要

FIELD: medicine.;SUBSTANCE: adherent fraction of mononuclear cells (MNC) is recovered from peripheral blood of patients suffering colorectal cancer and cultivated in the medium of certain composition for producing immature dendritic cells (DC). In the process of cultivation, the latter are added with a tumour antigen (autologous tumour cell lysate), and then rhTNF-α for maturation of the immature DC. The produced mature antigen-activated DC are cultivated with the non-adherent fraction of the MNC in the presence of the proinflammatory cytokines rhIL-12 and rhIL-18.;EFFECT: use of the method of generating the antigen-specific cytotoxic cells with anticancer activity provides high cytotoxicity of anticancer cells, and as a consequence the efficiency of a T-helper type 1 immune response on colorectal cancer antigens.;1 tbl