METHOD OF EXAMINING PEROXIDASE ACTIVITY OF BLOOD PLASMA HAEMOGLOBIN

摘要

FIELD: medicine.;SUBSTANCE: substratum o-dianisidine is introduced in blood plasma diluted in phosphate-citrate buffer (pH 5.5). After hydrogen peroxide added in the concentration of 2 mM, the substratum oxidation rate is evaluated by sepectrophotometry in the presence of the myeloperoxidase inhibitor - 4-aminobenzoic acid hydrazide. Peroxidase activity of blood plasma haemoglobin is described as the substratum o-dianisidine oxidation rate.;EFFECT: method enables fast and reliable evaluation of haemoglobin contribution to total peroxidase activity of blood plasma that is applicable in prediction of the developing diseases and choosing a therapeutic approach.;2 dwg, 1 tbl, 3 ex