REASSORTANT RN2/57-HUMAN A(H7N2) INFLUENZA VIRUS STRAIN FOR NEURAMINIDASE ANTIBODY ASSAY IN INFLUENZA INFECTION AND VACCINATION

摘要

FIELD: medicine.;SUBSTANCE: diagnostic RN2/57-human A(H7N2) influenza virus strain is produced by cross breeding of the sterile equine influenza virus A/horse/Prague/1/1956(H7N7) and the cold-adapted vaccine strain A/17/duck/Potsdam/86/92(H5N2) on the basis of the attenuation donor A/Leningrad/134/17/5 7(H2N2). The strain contains neuraminidase of the subtype 2 influenza virus A/Leningrad/134/17/1957(H2N2) and hemagglutinin of equine influenza virus A/horse/Prague/1/1956(H7N2). The RN2/57-human A(H7N2) strain replicates actively in growing chicken embryos at optimum temperature 33°C that enables collecting the viral material to be purified and concentrated.;EFFECT: invention may be used for detection neuraminidase antibodies of the N2 influenza virus in serums of experimental animals and volunteers with the use of solid-phase sialydase activity neuraminidase inhibition test.;1 dwg, 2 tbl