Sted - fluorescence light microscope with pulsed excitation, a continuous stimulation and temporally resolved registration of fluorescent light emitted spontaneously

摘要

Sted - fluorescence light microscope (1) with:– a pulsed excitation light source (4), the pulses of excitation light (3) onto a sample (2), wherein the excitation light (3) in the sample (2) to fluorescence fluorescent units containing excites and is focused on at least a focal region;– a continuous de-excitation light source (11), the continuous de-excitation light (12) on the sample (2), wherein the de-excitation light (12) the excited fluorescent units of the sample and a zero point in the at least has a focal region;– a detector (10), of the of the excited fluorescent units in the sample (2) spontaneously emitted fluorescent light (9) after each pulse of the excitation light (3) of the excitation light source (4) and in an overlapping manner with the application of the de-excitation light (12) by means of the de-excitation light source (11) is recorded;characterized in that the detector the of the excited fluorescent units spontaneously emitted fluorescent light (9) with a time resolution of between successive pulses of the excitation light (3) of the excitation light source (4) is recorded.