METHOD FOR TESTING PHOTOTOXICITY WITH HUMAN RETINAL PIGMENT EPITHELIAL CELL

摘要

PROBLEM TO BE SOLVED: To provide a method for testing phototoxicity, by which the phototoxicity expression ability of a test substance can effectively be tested.;SOLUTION: The method for testing phototoxicity is characterized by including the following processes (1)-(4), and the like: (1) The first process for contacting human retinal pigment epithelial cells with a test substance; (2) the second process for culturing the human retinal pigment epithelial cells made contact with the test substance in the first process, while irradiating it with artificial light containing a wavelength distribution in a range of 340-380 nm within 24 hours after the start of the contact in the first process; (3) the third process for recovering the human retinal pigment epithelial cells cultured in the second process and measuring the cytotoxicity of the recovered cultured cells or an index value correlated thereto; and (4) the fourth process for evaluating the presence or absence of the phototoxicity-expressing ability of the test substance or an extent thereof by the measurement result of the third process to test the phototoxicity expression ability of the test substance.;COPYRIGHT: (C)2013,JPO&INPIT