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Removal method of mycoplasma contamination of cell and cell culture method for mycoplasma contamination prevention
Removal method of mycoplasma contamination of cell and cell culture method for mycoplasma contamination prevention
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机译:细胞支原体污染的去除方法及预防支原体污染的细胞培养方法
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摘要
Strains that produce Amphitheatre di Nord, the present invention is obtained by culturing the strain Amphitheatre di nium-Kurebushi belonging dinoflagellates in (Dinoflagellates) (Amphidinium klebsii), Amphitheatre de-nium-Karutere such as (Amphidinium carterae), preferably by incubating the cells using the fractions obtained from the extract or extracts of the cells were cell culture method for preventing mycoplasma contamination potential; Use amphitheater di Nord derivatives by decide to use, culturing cells contaminated by mycoplasma, there is provided a method of removing the mycoplasma contamination from cells contaminated with mycoplasma. The present invention also provides a method of removing the mycoplasma contamination involves the removal of cell mass and enzyme treatment with a single solution for cell separation, the cells contaminated with mycoplasma.
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机译:通过在(Dinoflagellates)(Amphidinium klebsii),Amphitheater de-nium-Karutere(如(Amphidinium Carterae))中培养属于Dinoflagellates的Dinoflagellates的Amphitheater di dium-Kurebushi菌株来生产本发明的Nord露天剧场。使用从细胞提取物中获得的馏分或细胞提取物进行细胞培养的方法,以预防支原体污染的可能性;通过决定使用圆形剧场二诺德衍生物,培养被支原体污染的细胞,提供了一种从被支原体污染的细胞中去除支原体污染的方法。本发明还提供了一种去除支原体污染的方法,该方法包括去除细胞团并用单一溶液进行酶处理以进行细胞分离,所述细胞被支原体污染。
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