Rapid identification method of drug resistance genes using malaria parasite artificial chromosome and a recombinant malaria parasite production method

摘要

Using protozoan artificial chromosomes, the present invention relates to a rapid method of identifying drug resistance gene. Specifically, using an in vitro culture system of the blood stage parasites by preparing a recombinant malaria parasites by using the malaria parasite artificial chromosomes, using red blood cells or inoculated into non-human mammals, the recombinant in the presence of drug relates to a method of survival as an indicator of malaria parasite, screening for drug resistance gene. Furthermore, by directly introducing foreign genes at high efficiency Plasmodium, the invention is directed to a method of making a recombinant malaria parasites.