MTHFR gene amplification primer set, and its use MTHFR gene amplification reagent containing it

摘要

To provide a primer set for amplifying specifically target regions of the MTHFR gene by nucleic acid amplification method. (1), and a primer set including a primer F1 and R1 primers, I use a primer set containing (2) a primer set including a primer and the R2 primer and the F2. Thus, for example, at the same time, it is possible to amplify the target regions of two, each containing a portion generated polymorphism of two MTHFR gene in the same reaction mixture. (F1) base length is 20 to 28 bases in length, in the nucleotide sequence shown in SEQ ID NO: 1, 18 to 26 bases in length (R1) base length oligonucleotides and the 3 'end of the guanine nucleotide number 8715 (g) of It is, in the nucleotide sequence shown in SEQ ID No. 1, complementary oligonucleotides (F2) base length is 26-36 bases long oligonucleotide to the 5 'end cytosine at nucleotides 8817 and (c), and SEQ ID NO: In the nucleotide sequence shown in, oligonucleotides 3 'terminal guanine at nucleotides 10590 and (g) (R2) base length is 22-34 bases in length, in the nucleotide sequence shown in SEQ ID NO: 1, the nucleotide numbers 10695 The oligonucleotide complementary to the oligonucleotide to the 5 'end (c) cytosine