PRIMER SET FOR AMPLIFICATION OF MTHFR GENE, MTHFR GENE AMPLIFICATION REAGENT COMPRISING SAME, AND USE OF SAME

摘要

[summary] The nucleic acid primers to amplify specifically the goal area of the MTHFR gene amplification by law provides. A primer set comprising the F1 primer and R1 primer (1), and, using a primer set comprising a primer set (2) comprising a primer and R2 primer of F2. Thus, for example, at the same time in the same reaction solution, the part that causes the two types of polymorphism of MTHFR gene may amplify the target region including two each. (F1) The base length is 20-28 bases in length, in the nucleotide sequence shown in SEQ ID NO: 1, guanine (g) of 8715 nucleotides with an oligonucleotide to the 3 'terminal nucleotide (R1) and a length of 18-26 nucleotide bases in length, in the nucleotide sequence shown in SEQ ID NO: 1, nucleotides complementary oligonucleotide to the oligonucleotide which cytosine (c) of the base number 8817 to a 5 'terminal (F2) and the base length of 26 to 36 bases in length, in the nucleotide sequence shown in SEQ ID NO: 1, guanine (g) of the oligo-nucleotides 10590 to the 3 'terminal nucleotide (R2) and a length of 22-34 nucleotide bases in length, in the nucleotide sequence shown in SEQ ID NO: 1, oligo-cytosine (c) of the base numbers 10695 to a 5 'terminal nucleotide complementary to the oligo-nucleotide ;