Raman instrument for measuring a weak signal in the presence of a strong fluorescence background

摘要

The method of measuring a chemical concentration in tissue is determined in two stages. First, a step of generating a first light irradiating portion of the tissue using the light of the first, the steps of capturing a first reflected light from the tissue, the reflected light of the first the method comprising the steps of directing a plurality of optical sensors, and measuring the wavelength of light the optical sensor is different, the steps of the wavelength is close to the wavelength of the predicted Raman shift wavelength for chemicals in the tissue, the and a step of obtaining a measured value from the optical sensor. Secondly, a step of generating a second light, illuminating the part of the tissue using the light of the second, the steps of capturing a second reflected light from the tissue, the reflected light of the second the method comprising the steps of directing a plurality of optical sensors, and measuring the wavelength of light the optical sensor is different, the steps of the wavelength is close to the wavelength of the predicted Raman shift wavelength for chemicals in the tissue, the and a step of obtaining a measured value from the optical sensor.