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Colorimetric Device for Detecting, in an Aqueous Solution of Interest, Hydrolytic Enzymatic Activity with Regard to at Least One Polymer of Interest

机译:用于在目标水溶液中检测水解酶活性的比色装置,至少要检测一种目标聚合物

摘要

This invention concerns a colorimetric device for detecting, in an aqueous solution of interest, a hydrolytic enzymatic activity with regard to at least one polymer of interest.;This device (1) includes (i) a substrate (2) and (ii) a transparent detection layer (3), including the said polymer of interest.;This detection layer (3) is adapted so that, on the one hand, after application of the said aqueous solution of interest, when it is deprived of said hydrolytic enzymatic activity, it preserves the said first thickness e, and on the other, after the application of the said aqueous solution of interest, when it includes the said hydrolytic enzymatic activity, it has a second thickness e′, thinner than the said first thickness e,;and the said first thickness e and/or the said second thickness e′ of the said detection layer (3) are adapted to generate a colour by an optical interference phenomenon caused by the recombining of the light beams reflected at the interfaces (4, 5) of the said detection layer (5).
机译:本发明涉及用于在目标水溶液中检测至少一种目标聚合物的水解酶活性的比色装置。该装置( 1 )包括(i)底物( 2 )和(ii)包括上述目标聚合物的透明检测层( 3 )。该检测层( 3 )为适用于:一方面,在施用所述目的水溶液之后,当其失去所述水解酶活性时,其保留所述第一厚度e;另一方面,在施用所述水溶液之后感兴趣的是,当其包括所述水解酶活性时,其具有比所述第一厚度e薄的第二厚度e';以及所述检测层的所述第一厚度e和/或所述第二厚度e'( 3 )适于通过光学干涉现象产生颜色,该光学干涉现象是由反射的光束的重组引起的。所述检测层( 5 )的界面( 4、5 )。

著录项

  • 公开/公告号US2013115643A1

    专利类型

  • 公开/公告日2013-05-09

    原文格式PDF

  • 申请/专利权人 BERNARD CATHALA;CAROLE CERCLIER;

    申请/专利号US201113808861

  • 发明设计人 BERNARD CATHALA;CAROLE CERCLIER;

    申请日2011-07-06

  • 分类号C12Q1/34;

  • 国家 US

  • 入库时间 2022-08-21 16:47:04

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