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COLORMETRIC DEVICE FOR DETECTING, IN AN AQUEOUS SOLUTION OF INTEREST, HYDROLYTIC ENZYMATIC ACTIVITY WITH REGARD TO AT LEAST ONE POLYMER OF INTEREST

机译:用于在水溶液中检测相对于至少一种兴趣聚合物的水解酶活性的比色装置

摘要

This invention concerns a colorimetric device for detecting, in an aqueous solution of interest, a hydrolytic enzymatic activity with regard to at least one polymer of interest. This device (1) includes (i) a substrate (2) and (ii) a transparent detection layer (3), including the said polymer of interest. This detection layer (3) is adapted so that, on the one hand, after application of the said aqueous solution of interest, when it is deprived of said hydrolytic enzymatic activity, it preserves the said first thickness e, and on the other, after the application of the said aqueous solution of interest, when it includes the said hydrolytic enzymatic activity, it has a second thickness e′, thinner than the said first thickness e, and the said first thickness e and/or the said second thickness e′ of the said detection layer (3) are adapted to generate a color by an optical interference phenomenon caused by the recombining of the light beams reflected at the interfaces (4, 5) of the said detection layer (5).
机译:本发明涉及用于在目标水溶液中检测关于至少一种目标聚合物的水解酶活性的比色装置。该装置(1)包括(i)衬底(2)和(ii)透明检测层(3),其包括所述感兴趣的聚合物。将该检测层(3)调整为,一方面,在施加所述目的水溶液之后,当剥夺了所述水解酶活性时,其保留了所述第一厚度e,另一方面,保留了第一厚度e。在施用所述目的水溶液时,当其包括所述水解酶活性时,其具有第二厚度e和prime;其比所述第一厚度e薄,并且所述第一厚度e和/或所述第二厚度e和prime;所述检测层(3)的光束适于通过由在所述检测层(5)的界面(4、5)处反射的光束的重组而引起的光学干涉现象来产生颜色。

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