Method for producing complex of biotin-labeled Cypridina (Cypridina noctiluca) luciferase with streptoavidin and method for stabilizing the same

摘要

The present invention relates to a method for producing Cypridina luciferase labeled with hydrophilic biotin, characterized in that a biotin reagent containing a polyalkylene glycol structure as a spacer is reacted with Cypridina luciferase, and biotin-labeled Cypridina luciferase wherein a sugar chain in Cypridina luciferase has been biotinylated.