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Method for detecting binding interactions between membrane proteins and analytes in a homogenous assay
Method for detecting binding interactions between membrane proteins and analytes in a homogenous assay
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机译:在同质测定中检测膜蛋白与分析物之间结合相互作用的方法
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摘要
Devices and methods for detecting interaction between components associated with lipid membranes and analytes are described herein. In certain methods, a surface of a compartment of a device is coated with a material that attaches to lipid membrane from a sample. An analyte is introduced and interaction is detected, for example, by back scattering interferometry. In another method, a surface is passivated with a material that does not bind a lipid membrane. A sample is introduced that contains a membrane comprising a component for testing and an analyte. Interaction is detected. In a third method, a channel is filled with a first liquid that does not comprise a lipid membrane. A bolus of a second liquid that comprises a membrane comprising a component for testing and an analyte is introduced under laminar flow conditions so that a leading edge of the second liquid does not completely displace the first liquid in a sensing area that is interrogated by optical methods, for example, a laser. Interaction between the analyte and the component is detected.
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