Method for the production of insulin-1-like growth factor conjugates and poly (ethylene glycol)

摘要

Invention Patent: "METHOD FOR PRODUCTION OF INSULIN-1 AND POLY (ETHYLENE GLYCOL) GROWTH FACTOR CONJUGATES". A method for producing a lysine-pegylated IGF-I or lysine-pegylated IGF-I variant, said variant comprising one or two amino acids selected from the group consisting of lysine 27, 65 and / or 68 independently substituted by another amino acid polar, characterized in that it cultivates a prokaryotic host cell comprising an expression vector containing a nucleic acid encoding a fusion protein comprising said IGF-I or N-terminally linked IGF-I variant to a C-terminal of a propeptide, said propeptide has its C-terminus terminated with amino acids -Y-Pro, where Y is selected from the group consisting of Pro, Pro-Ala, Pro-Gly, Pro-Thr, Ala-Pro, Gly-Pro, Thr -Pro, Arg-Pro, or Pro-Arg-Pro, recovering and PEGylating said fusion protein, cleaving said IgA protease-pegylated fusion protein, and recovering said PEGylated IGF-I or IGP -ylated IGF-I variant . Pegylated IGF-I or IGF-I variant is useful for the treatment of neurodegenerative disorders such as Alzheimer's disease.