alpha amylase inhibitor mutants isolated from phaseolus vulgaris with insect pest control properties, compositions containing such mutants and method of use thereof

摘要

ALPHA INHIBITORS MUTANTS ISOLATED Phasoolus vulgaris WITH PROPERTIES TO CONTROL PEST INSECTS, COMPOSITIONS CONTAINING SUCH MUTANTS AND METHOD OF USE. The present invention relates to the field of pest insect control using methods and compositions comprising mutant alpha-amylase inhibitor analogs (? AIs). More specifically, the invention provides novel Î ”AI mutant analog molecules for pest insect control, particularly cotton boll weevil (Arnhonomus grandis), showing partial or total reduction in the amylolytic activity of digestive enzymes present in the intestinal lumen. Insect Also aspects of the invention are gene constructs containing nucleic acid molecules coding for alpha-amylase inhibitors, methods for heterologous expression of the new molecules in active form, as well as their use in insect pest control. The invention also provides synthetic analog genes which are optimized for transformation and expression in plants.