Constructions comprising a polynucleotide molecules encoding a heterologous acetilxiliano esterase; method to design a plant that has a reduced acetylation in cell walls; and a method for producing biofuel.

摘要

A method of engineering a plant having reduced acetylation in a cell wall is disclosed. The method comprising expressing in the plant cell wall at least one isolated heterologous polynucleotide encoding an acetylxylan esterase (AXE) enzyme under the transcriptional control of a developmentally regulated promoter specifically active in the plant cell wall upon secondary cell wall deposit, thereby engineering the plant having reduced acetylation in the cell wall.